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Original Article

The Effect of Nutrition Education Program on Overweight/Obese Patients with Non-Alcoholic Fatty Liver Disease: a Single-Blind Parallel Randomized Controlled Trial

Arman Arab1orcid, Amir Hadi2orcid, Seyedeh Parisa Moosavian3orcid, Nahid Rafie3orcid, Hossein Hajianfar4,5orcid
Clinical Nutrition Research 2019;8(3):238-246.
Published online: July 29, 2019

1Department of Community Nutrition, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.

2Halal Research Center of IRI, FDA, Tehran 314715311, Iran.

3Department of Clinical Nutrition, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.

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Correspondence to Hossein Hajianfar. Department of Nutrition, School of Nutrition and Food Sciences, Semnan University of Medical Sciences, Bassij Blvd., Semnan 3514799422, Iran. hossein.hajian2009@gmail.com
• Received: June 18, 2019   • Revised: July 20, 2019   • Accepted: July 22, 2019

Copyright © 2019. The Korean Society of Clinical Nutrition

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • The aim of this study was to assess the effects of a nutrition education program on overweight/obese patients with non-alcoholic fatty liver disease (NAFLD). This single-blind, parallel randomized controlled trial was conducted on 82 NAFLD patients (mean age 48.89 ± 9.85 years) in Isfahan, Iran. Patients were randomly allocated to receive nutrition education program combined with usual care or usual care alone for 2 months. Metabolic markers, including lipid profile, fasting blood glucose (FBG) and dietary intakes were obtained for each patient at baseline and at the end of the study. Nutrition education intervention resulted in a significant decrease compared with usual care in serum levels of total cholesterol (TC) and triglyceride (TG) (p < 0.05). However, our intervention did not lead to a significant improvement in FBG, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C), respectively (p > 0.05). Furthermore, energy and sugar intakes of patients were reduced significantly in response to our intervention (p < 0.05). Nutrition education intervention significantly reduced serum levels of TC and TG but did not affect other metabolic markers including FBG, LDL-C, and HDL-C. Also, it could reduce total energy and sugar intake. Thus, this study could be useful to use this educational program for NAFLD patients in order to influence their metabolic markers and dietary intakes.
  • Trial Registration
    IRCT Identifier: IRCT2014101811763N17
  • Keywords: Non-alcoholic fatty liver disease; Nutrition program; Clinical trial
Non-alcoholic fatty liver disease (NAFLD) is a major public health problem due to its prevalence as high as 25%–30% worldwide which is expected to rise in the near future [1]. Traditionally, it has been suggested that NAFLD is the “hepatic manifestation” of the metabolic syndrome [2]. NAFLD is linked with obesity and also encompasses a range of other liver pathologies including steatosis, nonalcoholic steatohepatitis (NASH), fibrosis, and cirrhosis. Furthermore, it has been reported that the incidence of hepatocellular carcinoma (HCC) could be increased in the presence of NAFLD up to 25% [3]. In addition to hepatic dysfunctions, other metabolic abnormalities have been increased paralleled with NAFLD such as type 2 diabetes mellitus (T2DM), cardiovascular disease and dyslipidemia [4]. Dyslipidemia is defined as the presence of one or more abnormal serum lipid concentration; total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) [5]. Since dyslipidemia is the main risk factor related to cardiovascular disease (CVD), sufficient attention is needed to lower dyslipidemia and its undesirable effects. Currently, various treatment options that target each aspect of the dyslipidemia pathogenesis are being explored and advocated, but recent guidelines encourage combination therapy for the management of multiple lipid abnormalities [6, 7]. In the other words, non-pharmacological therapy should be combined with pharmacotherapy.
The Adult Treatment Panel III placed major prominence on lifestyle modification intervention as an essential strategy for clinical management of people at risk for CVD, including individuals with dyslipidemia [8]. Lifestyle modification interventions try to change dietary and exercise behavior of individuals in order to improve their health status. The fundamental components of lifestyle modifications are to clarify healthy dietary patterns and encourage physical activity [9]. Despite clear data and recommendations for NAFLD patients with regard to the management of metabolic abnormalities, little information exists on the utility of diet as a treatment for metabolic abnormalities in NAFLD patients. We, therefore, sought to evaluate the effect of a 2-month nutrition education program on metabolic markers (TC, TG, LDL-C, HDL-C, and fasting blood glucose [FBG]) and dietary intake of overweight/obese NAFLD patients. The program was designed to improve metabolic parameters through dietary modification as a simple, cost-benefit and non-invasive method compared to available pharmacotherapy.
Design
This was a parallel group, single-blind randomized controlled trial comparing the effect of a nutrition education program with usual care in Non-alcoholic fatty liver disease (NAFLD) patients.
Participants
From September 2015 to June 2016, patients with suspect of NAFLD was consecutively evaluated in the fatty liver clinic of Sedighe Tahere, Isfahan Endocrine and Metabolism Research Center. Subjects were admitted directly to our fatty liver clinic or referred from other general medicine or gastroenterology clinics located in Isfahan city. Subjects found to have NAFLD were then invited to enter this trial. We included patients diagnosed with NAFLD by an expert physician using sonography and biomarkers with higher levels of both alanine aminotransferase and aspartate transaminase more than 31 in men and women) [10], aged 20–50 years old; characterized as overweight/obese (body mass index [BMI] ≥ 24.9 kg/m2) with willingness to participate in the study.
Exclusion criteria were as follows: histologic diagnoses of borderline steatohepatitis or cirrhosis, missing more than 2 education sessions, pregnancy or breastfeeding, alcohol consumption above 20 g/day in men and 10 g/day in women, evidence of other causes of liver disease including drug-induced liver disease, viral hepatitis, autoimmune liver disease, Wilson's disease, hemochromatosis, α-1 antitrypsin deficiency, human immunodeficiency virus positive, severe psychiatric condition, drug dependence or inability to provide informed consent.
All patients provided informed written consent. The study protocol conformed to the ethical guidelines of the 1975 Declaration of Helsinki and was approved by the Research Ethics Committee of Isfahan University of Medical Sciences (ethic number: 393583), and registered at Iranian Registry of Clinical Trials (registration number: IRCT2014101811763N17). In addition, the Consolidated Standards of Reporting Trials (CONSORT) statement guidelines were used to design current study [11].
Sample size
Sample size was calculated by using the sample size formula suggested for similar studies. Considering type 1 and 2 errors 5% (α = 0.05) and 20% (β = 0.2; power = 80%) and considering for a possible loss, we reached the sample size of 41 patients for each group [12].
Trial procedures
Of 327 patients who were invited to the fatty liver clinic, 82 participants fulfilling the inclusion and exclusion criteria were randomized in 1:1 ratio to participate in the nutrition education program (n = 41) or receive usual care (n = 41). To guarantee recruitment balance between the 2 groups and avoid possible risk for selection bias, a blocked randomization method with randomly permuted blocks sizes of 4 was conducted by the biostatistician. Treatment assignments were concealed in numbered sealed envelopes which were opened sequentially upon patient enrollment. Clinicians of fatty liver clinic and statisticians who analyzed results were blinded to the treatment assignment.
Intervention group
Patients randomized to the intervention group (n = 41) participated in a dietitian-led nutritional education. The patients attended dietary consultation sessions weekly for a total of 2 months. Participants were encouraged to increase the intake of fruits, vegetables, complex carbohydrate, low fat dairy, healthy fats, white meat and fish, and also avoid the intake of unhealthy fats and refined carbohydrates. Moreover, techniques for at-risk situations such as parties and festival celebrations were taught. Furthermore, in each session, the dietitian reviewed the patients' dietary practice and provided recommendations. Adherence to dietary intervention was assessed by weekly phone calls and dietary records. Educational contents of each session were prepared based on United States Department of Agriculture guidelines [13]. The goals of each session are illustrated in Table 1.
Table 1

Lesson plan of nutrition education based on health belief model

Table 1
Session Session title Content
1 NAFLD - Definition, risk factors, sign and symptoms of NAFLD.
- An overview of dietary guidelines for NAFLD patients.
- Weight loss plan and strategies.
2 Food groups - Introducing food guide pyramid and my plate.
- Explaining bread, cereal, rice & pasta group, and healthy choices.
- Explaining meat, poultry, fish, dry beans, eggs & nuts group, and healthy choices.
3 Food groups - Explaining fruit group and healthy choices.
- Explaining vegetable group and healthy choices.
- Explaining milk, yogurt & cheese group, and healthy choices.
4 Food groups - Explaining fat, different types of fat and healthy choices.
5 Food groups and treatment - Explaining fiber, different types of fiber and its benefits in NAFLD.
- A brief explanation of medical treatment of NAFLD.
6 Food groups - Explaining sugar, sweets & sugar sweetened products and their relation to NAFLD.
7 Food groups & exchange list - Explaining of exchange list and summarization of food groups
8 Physical activity and review - Explaining the importance of physical activity alongside with healthy dietary pattern.
- All of session material reviewed and summarized.
NAFLD, non-alcoholic fatty liver disease.
Control group
Patients randomized to the control group (n = 41) received routine care at the fatty liver clinic of Sedighe Tahere, Isfahan Endocrine and Metabolism Research Center. At baseline, a physician explained the laboratory test results and the natural history of NAFLD to the patients and then the patients were encouraged to reduce calorie, carbohydrate and fat intake by clinicians.
Data collections
All of the study variables including FBG, lipid profile, physical activity level, anthropometric measures, and dietary intakes were assessed at baseline and after 2 months of intervention. The information about dietary intakes was gathered using a 3-day food record questionnaire, and data was analyzed by nutritionist 4 software (First Databank, Hearst Corp., San Bruno, CA, USA). Participants were asked to record all of their intake during 3 days including 2 usual days and 1 weekend. Physical activity was evaluated by a physical activity record questionnaire which estimated the physical activity status based on metabolic equivalent (MET) tables [14]. Patients were asked to record all of their activities during 3 days including 2 usual days and 1 weekend. Body weight was measured to the nearest 0.1 kg after overnight fasting, without shoes and with minimal clothing by use of a digital scale using IOI 353 (Jawon, Seoul, Korea). Height was measured to the nearest 0.1 cm by using a stadiometer (Seca, Hamburg, Germany). BMI was calculated as weight in kilograms divided by height in meters squared.
Blood samples (10 mL) were collected after twelve hours of overnight fasting. Blood samples were immediately centrifuged (Avanti J-25; Beckman, Brea, CA, USA) at 3,500 rpm for 10 minutes to separate serum. Samples were then stored at −70°C before analysis. We used commercial kits to measure FBG, TG, TC, LDL-C, and HDL-C concentrations (Pars Azmoon Company, Tehran, Iran). The intra- and inter-assay coefficient of variations (CVs) for FBG were 2.0% and 3.5%, respectively. All inter- and intra-assay CVs for lipid profile measurements were < 5%. Measurements of glucose and lipids were performed in a blinded fashion in duplicates (before and after the intervention) at the same time, in the same analytic run, and in random order to reduce systematic error and inter-assay variability.
Statistical analysis
Continuous and categorical data were presented as mean ± standard error and percentage, respectively. Normality distribution of continuous data was evaluated using Kolmogrove-smirnov statistical test, and non-normality distributed data were subjected to natural logarithmic transformation. Within and between groups comparison in terms of quantitative data were conducted using paired samples t-test and analysis of covariance (adjustment was made for baseline values) while for categorical data generalized Mc-Nemar test and χ2 tests were used. All statistical analyses were performed using SPSS software version 15 (SPSS Inc., Chicago, IL, USA). A 2-sided p value of less than 5% was taken as statistically significant.
Four patients in the nutritional education group were excluded due to recurrent lumbar disk (n = 2), following an especial weight-loss plan (n = 1) and unwillingness to attend the program (n = 2). Eight patients in the control group were also excluded for the following reasons: not referring to perform secondary measurements (n = 6) and following an especial weight-loss plan (n = 2). A total of 69 participants (nutritional education [n = 36] and control [n = 33]) completed the trial (Figure 1).
Figure 1

Flow diagram of the study.

cnr-8-238-g001.jpg
Table 2 shows general characteristics of NAFLD patients who received either nutritional education in addition to usual care or just usual care. There was no significant difference between the two groups for age, educational level, sex, BMI, weight, physical activity, energy intake, and drugs used (p > 0.05). Mean (standard deviation) age, weight and BMI of participants were 48.89 (9.85) years, 83.70 (12.14) kg, and 32.18 (3.83) kg/m2, respectively.
Table 2

General characteristics of NAFLD patients who received either nutritional education in addition to usual care or just usual care

Table 2
Variables Intervention group (n = 41) Control group (n = 41) p value
Age (yr) 49.45 ± 1.46 48.23 ± 1.63 0.581*
Educational level 0.492†
Illiterate (%) 11.4 18.4
Elementary (%) 50 28.9
Diploma (%) 25 31.6
University education (%) 13.6 21.1
Sex, M/F (%) 27.3/72.7 36.8/63.2 0.351†
Weight (kg) 83.66 ± 1.77 83.75 ± 2.06 0.97*
BMI (kg/m2) 32.35 ± 0.60 31.98 ± 0.59 0.66*
Physical activity (MET) 34.15 ± 0.60 33.69 ± 0.72 0.62*
Energy intake (kcal) 2,350.67 ± 111.28 2,502.51 ± 100.58 0.39*
Calorie requirement (kcal) 2,020 ± 150.11 2,085 ± 150.46 0.76*
Metformin used (%) 31.8 27.8 0.69†
Glibenclamide used (%) 4.5 5.6 0.83†
Statins used (%) 22.7 11.1 0.17†
Gemfibrozil used (%) 2.3 2.8 0.88†
All values are means ± standard error except for educational level and gender presented as a percentage.
NAFLD, non-alcoholic fatty liver disease; BMI, body mass index; MET, metabolic equivalent.
*Calculated by independent t-test; †calculated by χ2 test.
Table 3 shows that nutritional education could significantly decrease serum levels of TC and TG (p < 0.05) compared to usual care. We did not find any significant effect of nutritional education on serum FBG, LDL-C, and HDL-C.
Table 3

Weight, lipid profile and FBG at the baseline and following an 8-week intervention in of NAFLD patients who received either nutritional education in addition to usual care or just usual care

Table 3
Variables Intervention group (n = 36) p value* Control group (n = 33) p value* p value†
Before After Before After
Weight (kg) 82.82 ± 1.93 81.05 ± 1.96 < 0.001 85.69 ± 2.14 85.05 ± 2.15 0.09 0.02
FBG (mg/dL) 116 ± 6.69 108.83 ± 5.03 0.009 110.14 ± 8 99.58 ± 2.57 0.17 0.25
TC (mg/dL) 215 ± 10.52 193.93 ± 11.62 0.007 186 ± 6.21 190 ± 6.01 0.33 0.04
TG (mg/dL) 218.27 ± 26.12 164.51 ± 14.47 0.003 186.48 ± 26.65 196.06 ± 20.95 0.04 0.002
LDL-C (mg/dL) 120.19 ± 6.67 109.62 ± 6.95 0.04 111.14 ± 5.64 114.54 ± 5.35 0.36 0.46
HDL-C (mg/dL) 46.48 ± 1.84 45.03 ± 1.31 0.61 43.38 ± 1.78 41.32 ± 1.37 0.02 0.12
Data are presented as means ± standard error.
FBG, fasting blood glucose; NAFLD, non-alcoholic fatty liver disease; TC, total cholesterol; TG, triglyceride; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.
*Calculated by paired t-test; †calculated by analysis of covariance, adjusted for baseline values and dietary energy intakes.
Dietary intakes of participants revealed that the amount of carbohydrates, protein and total fat, consumed by intervention group, decreased significantly compared to the placebo (p < 0.05) but the percentage of these macronutrients did not differ between the two groups (p > 0.05). Moreover, total energy and sugar intake of the nutrition education group reduced significantly compared to the usual care group (p < 0.05) (Table 4).
Table 4

Dietary intakes at the baseline and following an 8-week intervention in NAFLD patients

Table 4
Variables Intervention group (n = 36) p value* Control group (n = 33) p value* p value†
Before After Before After
Carbohydrate (g/day) 327.41 ± 19.23 240.58 ± 13.95 < 0.001 396.22 ± 38.47 343.26 ± 14.73 0.16 < 0.001
Protein (g/day) 84.98 ± 4.51 66.90 ± 4.17 < 0.001 97.04 ± 4.42 90.14 ± 3.51 0.09 0.001
Total fat (g/day) 74.77 ± 4.56 51.80 ± 3.03 < 0.001 78.67 ± 4.70 72.68 ± 3.80 0.09 < 0.001
Carbohydrate (%) 56.90 ± 1.33 57.03 ± 1.23 0.91 57.25 ± 1.17 57.80 ± 1.11 0.60 0.67
Protein (%) 14.62 ± 0.37 16.03 ± 0.56 0.01 15.32 ± 0.47 15.22 ± 0.52 0.85 0.10
Total fat (%) 28.46 ± 1.32 26.90 ± 1.05 0.16 27.41 ± 1.26 26.96 ± 1.07 0.64 0.62
Saturated fat (g/day) 19.18 ± 1.33 13.35 ± 0.78 < 0.001 21.19 ± 1.07 19.36 ± 0.87 0.07 < 0.001
Sugar (g/day) 71.93 ± 4.46 57.52 ± 3.33 0.009 107.53 ± 32.83 76.14 ± 5.49 0.34 0.008
Energy intake (kcal/day) 2,350.67 ± 111.28 1,729.43 ± 86.62 < 0.001 2,502.51 ± 100.58 2,347 ± 85.81 0.02 < 0.001
Data are presented as means ± standard error.
NAFLD, non-alcoholic fatty liver disease;
*Calculated by paired t-test; †calculated by analysis of covariance, adjusted for baseline values and dietary energy intakes.
Our study showed that a nutrition education intervention in NAFLD patients resulted in a significant decrease in serum TC and TG but did not affect other metabolic markers including FBG, LDL-C, and HDL-C. Also, we revealed that 2 months of nutrition education intervention could decrease total energy, sugar, carbohydrate, protein and fat intake of participants but this intervention did not change the percentage of consumed macronutrients.
Appropriate dietary changes are such an essential component in the management of NAFLD and its co-existing complications because of their demonstrated effects on glycemic control and lipoprotein profile [15]. Therefore, it is not surprising that biochemical profile of the participants was enhanced when attention was paid to the nutritional aspects of life. The reduction in the lipid profile was modest but significant since it was accomplished in a very short time period by a very straightforward and cost-effective intervention. The favorable changes in biochemical indices could be attributed to the dietary changes achieved from this nutrition education intervention. Although, the amount of macronutrient consumed by patients was reduced significantly, the percent of these macronutrients did not change which means that it was energy intake that showed the most alterations. On the other hand, our result showed that included patients consumed less energy with the same share of macronutrients. It should be mentioned that the short intervention duration (2 months) cannot be established to prove lifelong changes in eating habits, but does show the ability of the education program to influence on some aspects of the individuals' diet. So, the results of our study are comparable with other studies using similar methods, but less robust than those from studies that established a multidisciplinary lifestyle modification program for a longer period. Furthermore, it should be stated that some of our study participants' plasma glucose or serum lipoprotein profiles were in the normal range. However, even for individuals whose metabolic profiles are in the normal range, a further improvement is appreciated in terms of primary prevention of other complications such as T2DM, metabolic syndrome and CVD.
It has been suggested that weight reduction is the main underlying mechanism which could link nutrition education to metabolic indices' improvement. We reported in our previous study that our nutrition education intervention could reduce weight and BMI significantly [10]. Weight reduction has been accompanied by significant improvements in blood glucose and lipid abnormalities [16, 17]. Surprisingly, beneficial effects of weight loss may be even possible at high post-treatment BMIs (> 30 kg/m2) [18, 19]. Furthermore, our intervention reduced sugar intake of participants. Ambrosini et al. [20] demonstrated that higher sugar intake as sugar sweetened beverages was associated with higher levels of serum TG which is in line with our findings. It has been suggested that the association between sugar intake and lipid abnormalities might be independent of BMI [20]. There is mechanisms which link higher sugar intake to cardio-metabolic risk factors. Higher sugar intake without compensatory reduction in energy intake has resulted in positive energy balance and obesity [21]. Furthermore, sugar with its rapidly absorbable properties led to a high dietary glycemic load, which is linked with insulin resistance, inflammation and increased cardiovascular risk [22, 23].
Possible limitations of the study should be considered when interpreting the data and planning future research. Collecting dietary intake data via self-report is mostly accompanied with reporting bias. On the other hand, although we used 3-day food records as a gold standard method of dietary assessment, our included participants were overweight/obese which have been proved to underestimate their dietary intakes. Another point is that more patients might be included, if available, in future studies even though the sample size was adequate according to power calculations and scientific standards for research. However, there are important strengths to our study as well. The method of our nutrition education intervention is simple and low-priced that could be used by clinicians in private or public clinics.
According to what was discussed, we found that nutrition education intervention could significantly reduce serum levels of TC and TG but did not affect other metabolic markers including FBG, LDL-C, and HDL-C. Also, it could reduce total energy, saturated fat and sugar intake. Thus, this educational program could be useful for NAFLD patients in order to improve their metabolic markers and simultaneously dietary intakes as an easy, cost-benefit and non-invasive approach. This program could be used by clinicians in private clinics or for public health education. Furthermore, we need more clinical trials with more sample size and duration to draw a firm link between nutritional changes and metabolic markers’ improvements.
Isfahan University of Medical Scienceshttps://doi.org/10.13039/501100003970

Funding: This study was supported by Isfahan University of Medical Sciences, Isfahan, Iran.

Conflict of Interest: The authors declare that they have no competing interests.

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The Effect of Nutrition Education Program on Overweight/Obese Patients with Non-Alcoholic Fatty Liver Disease: a Single-Blind Parallel Randomized Controlled Trial
Clin Nutr Res. 2019;8(3):238-246.   Published online July 29, 2019
DOI: https://doi.org/10.7762/cnr.2019.8.3.238
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The Effect of Nutrition Education Program on Overweight/Obese Patients with Non-Alcoholic Fatty Liver Disease: a Single-Blind Parallel Randomized Controlled Trial
Clin Nutr Res. 2019;8(3):238-246.   Published online July 29, 2019
DOI: https://doi.org/10.7762/cnr.2019.8.3.238
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The Effect of Nutrition Education Program on Overweight/Obese Patients with Non-Alcoholic Fatty Liver Disease: a Single-Blind Parallel Randomized Controlled Trial
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Figure 1 Flow diagram of the study.

Figure 1

The Effect of Nutrition Education Program on Overweight/Obese Patients with Non-Alcoholic Fatty Liver Disease: a Single-Blind Parallel Randomized Controlled Trial
Table 1 Lesson plan of nutrition education based on health belief model

NAFLD, non-alcoholic fatty liver disease.

Table 2 General characteristics of NAFLD patients who received either nutritional education in addition to usual care or just usual care

All values are means ± standard error except for educational level and gender presented as a percentage.

NAFLD, non-alcoholic fatty liver disease; BMI, body mass index; MET, metabolic equivalent.

*Calculated by independent t-test; †calculated by χ2 test.

Table 3 Weight, lipid profile and FBG at the baseline and following an 8-week intervention in of NAFLD patients who received either nutritional education in addition to usual care or just usual care

Data are presented as means ± standard error.

FBG, fasting blood glucose; NAFLD, non-alcoholic fatty liver disease; TC, total cholesterol; TG, triglyceride; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.

*Calculated by paired t-test; †calculated by analysis of covariance, adjusted for baseline values and dietary energy intakes.

Table 4 Dietary intakes at the baseline and following an 8-week intervention in NAFLD patients

Data are presented as means ± standard error.

NAFLD, non-alcoholic fatty liver disease;

*Calculated by paired t-test; †calculated by analysis of covariance, adjusted for baseline values and dietary energy intakes.

Table 1

Table 2

Table 3

Table 4

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